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Отдел экспериментальной терапии

Основан как отдел патологии и терапии в 1944 году (1944 - 1970 - заведующий академик АМН СССР А.И. Черкес). С 1970 по 1992 гг. отделом руководила профессор, доктор медицинских наук Б.С. Бравер-Чернобульская. С 1992 по 2007 гг. - академик АМН Украины, проф. А.В. Стефанов.

Соловйов А.І.

Заведующий отделом экспериментальной терапии (с 2008 г.) д-р мед. наук, проф., дважды лауреат Государственной премии Украины в области науки и техники Соловьев Анатолий Иванович

 

Тел.: +38(044)536-13-41, +38(044)456-02-88
E-mail: tonysolpharm@gmail.com

 

Научные направления:

 

  • исследование роли факторов эндотелиального происхождения в развитии нарушений сердечно-сосудистой системы и обоснование путей их фармакологической коррекции;
  • разработка новых методов лечения с помощью липосомальных препаратов;
  • клеточные и молекулярные механизмы развития артериальной гипертензии различного генеза и разработка методов коррекции с помощью феномена РНК-интерференции.

 

Методы научных исследований:

 

  • регистрация сократительной активности интактных и химически сканированных гладких мышц;
  • метод сахарозного мостика;
  • patch-clamp техника для регистрации трансмембранных ионных токов;
  • измерение выделения оксида азота;
  • измерение внутриклеточного кальция флуоресцентным методом;
  • изучение кардио-гемодинамики in vivo;
  • изучение биодоступности и биоэквивалентности лекарственных средств в условиях in vitro использования клеточной линии Сасо-2;
  • РНК-интерференция.

 

Достижения / Международные научные проекты:

 

  • Biomimetics of NO-Chemistry: Mechanisms of NO-Release, Oxidative Fate of NO and Design a New Generation of NO Prodrugs and Inhibitors (CNRS, Toulouse, France, 1993-1995).
  • The Identification of O2-Sensitive Membrane Ionic Channels in Neuronal and Vascular Smooth MuscleCells: Fundamental basis to Design a New Generation of Antihypoxic Drugs (INTAS, UK/London, Lund/Sweden, 1995-1997).
  • Changes in Vasorelaxant Activity of Therapeutic Nitric Oxide Donors After Whole Body irradiation and its relevant to Chernobyl Disaster (Wellcome Trust, Glasgow, UK, 1999-2001).
  • Mechanisms of Vascular Contractile Dysfunction Induced by Ionizing Irradiation: Special Relation to Change in Myofilament Calcium Responsivenes (CRDF/NIH, USA, 2003 -2004)
  • Enhanced Vascular Contractility in Diabetes: the Role of Calcium Sensitization. Royal Society, Belfast University, UK, 2006 – 2007.
  • BKCa Cnannels and Endothelium-Dependent  Vascular Responses Under Ionized Non-Fatal Whole-Body Irradiation: New Insight into Radiation-Induced Vascular Lesions. British Physiological Society Centres of Excellence Award Scheme, UK, 2007-2009.

 

Наиболее важные публикации:

  1. Soloviev A., Braquet P. Platelet-Activating Factor Induces Contraction of Saponin-Skinned Smooth Muscle of Coronary Artery //Lipids.- 1991.- 26.- р. 1273-1276.
  2. Soloviev A., Bershtein S. The Contractile Apparatus in Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats Possess Increased Calcium Sensitivity: the Possible Role of Protein Kinase C.//J. of Hypertens.-1992.-10.- р. 131-136.
  3. Soloviev A., Braquet F. Platelet-Activating Factor - a Potent Endogenous Mediator Responsible for Coronary Vasospasm // News in Physiol. Sci.-1992, 7, 166-172.
  4. Soloviev A. et al. Phospholipid Vesicles (Liposomes) Restore Endothelium-Dependent Cholinergic Relaxation in Thoracic Aorta from Spontaneously Hypertensive Rats // J. of Hypertens.- 1993.-№11.- р. 623-637.
  5. Soloviev A., Bazilyuk O. Evidence for Decrease in Myofilament Responsiveness to Ca2+  During Hypoxia in Spontaneously Active Vascular Smooth Muscle in Rats //  Experim. Physiol.- 1993.-№18.- р. 395-402.
  6. Soloviev A., Braquet P. The Similarity in Action of Hypoxia and Platelet-Activating Factor on Smooth Muscle Cells of Coronary Arteries: Possible Explanation for Hypoxic Coronary Spasm Development. In: Mediators in the Cardiovas.System: Regional Ischemia. (eds. K. Schror, C.R. Pace-Asciak).- Birkhauser Verlag, Basel-Boston-Berlin.- 1995.-р. 275-282.
  7. Soloviev A. et al. Changes in Membrane Ionic Permeability and Related Contractile Responses in Vascular Smooth Muscle at Hypoxia // Pathophysiology.- 1996.- № 3.- р. 11-20.
  8. Soloviev A. et al. Evidence for the Involvement of Protein Kinase C in Depression of Endothelium-Dependent Vascular Responses in Spontaneously Hypertensive Rats // J. Vasc. Res.- 1998.- № 35.- р. 325-331.
  9. Soloviev A. et al. Depression of Endothelium-Dependent Relaxation Despite Normal Release of Nitric Oxide in the Aorta of Spontaneously Hypertensive Rats: Possible Role of Protein Kinase C. In: Endothelium-Derived Hyperpolarizations / Ed. P. Vanhoutte, Harwood Academic Publishers, 1999.- р. 289-296.
  10. Soloviev A. et al.The EDHF-Dependent, but not the NO-Dependent Component of the Acetylcholine-Induced Relaxation of the Rabbit Aorta is Resistant to Ionised Radiation. In: EDHF-2000 / Ed. P.Vanhoutte.- Taylor&Francis, London, New York, 2001.- р. 400-409.
  11. Lehen’ky V, Zelensky S., Stefanov A. and Soloviev A. Effects of Nitric Oxide Donors on a Type Vascular Smooth Muscle Preactivation // Cardiovasc.Toxicol.- 2002.- № 2.- р. 151-160.
  12. Soloviev A. et al. Arrhythmogenic Peroxynitrite-induced Alterations in Mammalian Heart Contractility and Its Prevention with Quercetin-Filled Liposomes // Cardiovasc.Toxicol.- 2002.- № 2.- р. 129-140.
  13. Soloviev A. et al. Saline Containing Phosphatidylcholine Liposomes Possess the Ability to Restore Endothelial Function Damaged Resulting from g-irradiation // J. Physiol. Pharmacol.- 2002.- V.53.- N 4.- р. 121-132.
  14. Soloviev A., et al. Mechanisms of Endothelial Dysfunction After Ionized Irradiation: Selective Impairment of the Nitric Oxide Component of Endothelium-Dependent Vasodilatation // Br. J. Pharmacol.- 2003.- V. 138.- р. 837-844.
  15. Soloviev A. et al. Mechanisms of Selective Impairment of Nitric Oxide-Mediated Endothelium-Dependent Vasodilatation Following Ionizing Irradiation // Neurophysiology.- 2003.- V. 35.-№ 3-4.- р. 276-283.
  16. Soloviev A. et al. Nitric Oxide Relaxes Rat Tail Artery Smooth Muscle by Cyclic GMP-Independent Decrease in Calcium Sensitivity of Myofilaments // Cell Calcium.- 2004.- V. 36- р. 165-173.
  17. Soloviev A. et al. Ionizing Rdiation Alters Myofilament Calcium Sensitivity in Vascular Smooth Muscle: Potential Role for Protein Kinase C // Am.J.Physiol. Integr. Comp.Physiol.- 2005.- V.289.- р. 755-762.
  18. Tishkin S, Rekalov V, Ivanova I, Moreland R,  and Soloviev A. Ionizing non-fatal whole-body irradiation inhibits Ca2+ -dependent K+ channels in endothelial cells of rat coronary artery: Possible contribution to depression of endothelium-dependent vascular relaxation // Int. J. Radiat Biol..-2007.- V. 83:3.- р. 161-169.
  19. Anatoly Soloviev, Sergey Tishkin, Irina Ivanovа et. al, Functional and molecular consequences of ionizing irradiation on large conductance Ca2+ - activated K+ channels in rat aortic smooth muscle cells // Life Sciences.- 2009.- V. 84.- р. 164-171.
  20. Soloviev A. et al. Quercetin-filled phosphatidylcholine liposomes restore abnormalities in rat thoracic aorta BKCa channel function following ionizing irradiation // Acta Physiol. Sinica.- 2009.- V. 61 (3).- р. 201-210.
  21. Soloviev A. et al. Electrophysiological and contractile evidence the ability of human mesenchymal stromal stem cells to correct vascular malfunction in rats after ionizing irradiation //  The J. of Physiol. Sci. (Japan).- 2010.- V. 60.- № 2.- р. 161-172.
  22. Kizub I,, Pavlova O,, Ivanova I., Soloviev A.  Protein kinase C-dependent inhibition of BKCa current in rat aorta smooth muscle cells following g-irradiation.Int. J. Radiat. Biol., 2010. 86, 291-299.
  23. Kizub I., Pavlova O., Johnson C., Soloviev A. Zholos A.  Rho kinase and protein kinase C involvement in vascular smooth muscle myofilament calcium sensitization in arteries from diabetic rats // Br J Pharmacol.- 2010.- V. 159.- р. 1724-1731.
  24. Kyrychenko S.,  Tishkin S., Dosenko V.,  Ivanova I.,  Novokhatska T., Soloviev A.  The BKCa channels deficiency as a possible reason for radiation-induced vascular hypercontractility //  Vasc. Pharmacol.- 2012.-V.56.- р. 142-149.
  25. Soloviev A., Bondarenko A., Kizub I.  Selective glycolysis blockade in guinea pig pulmonary artery and aorta reverses  contractile and electrical responses to acute hypoxia // Vasc. Pharmacol.- 2012.- V. 57.- р. 119-123.